Journal: Journal of Cancer
Article Title: Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma
doi: 10.7150/jca.92616
Figure Lengend Snippet: The results of qRT-PCR and Western blot for 10 ARGs genes. (A) The expression of BCL2L10, MELK, ERO1A, KRT8, KRT18, DDIT4, PERP, BTK, CX3CR1 and DAPK2 determined by qRT-PCR in A549 and PC9 cells; (B-C) The expression of BCL2L10, MELK, ERO1A, KRT8, KRT18, DDIT4, PERP, BTK, CX3CR1 and DAPK2 determined by Western blot in A549 and PC9 cells. P values were showed as: * P < 0.05.
Article Snippet: Primary antibodies against BCL2L10 (GeneTex, USA, 111872,1:1000 dilution), MELK (Proteintech, USA, 11403-1-AP, 1:1000 dilution), ERO1A (Proteintech, USA, 12007-1-AP, 1:1000 dilution), KRT8 (Proteintech, USA, 27105-1-AP, 1:1000 dilution), KRT18 (Proteintech, USA, 10830-1-AP,1:1000 dilution), DDIT4 (Proteintech, USA, 10638-1-AP, 1:1000 dilution), PERP (GeneTex, USA, 135223,1:1000 dilution), BTK (Proteintech, USA, 21581-1-AP, 1:1000 dilution), CX3CR1 (Proteintech, USA, 13885-1-AP, 1:1000 dilution), DAPK2 (Proteintech, USA, 20048-1-AP, 1:1000 dilution), and β-actin (ImmunoWay, TX, YM3028, 1:1000 dilution).
Techniques: Quantitative RT-PCR, Western Blot, Expressing